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The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles

Zhang, Qi ; Li, Yulong ; Tsien, Richard W

Science (New York, N.Y.), 13 March 2009, Vol.323(5920), pp.1448-53 [Peer Reviewed Journal]

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  • Title:
    The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles
  • Author: Zhang, Qi ; Li, Yulong ; Tsien, Richard W
  • Subjects: Membrane Fusion ; Synaptic Transmission ; Neurons -- Physiology ; Synaptic Vesicles -- Physiology
  • Is Part Of: Science (New York, N.Y.), 13 March 2009, Vol.323(5920), pp.1448-53
  • Description: Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.
  • Language: English
  • Identifier: E-ISSN: 1095-9203 ; PMID: 19213879 Version:1 ; DOI: 10.1126/science.1167373

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