skip to main content

Characterization of 13 microsatellite markers for Calochortus gunnisonii (Liliaceae) from Illumina MiSeq sequencing

Fuller, Ryan S. ; Frietze, Seth ; Mcglaughlin, Mitchell E.

Applications in Plant Sciences, August 2015, Vol.3(8), pp.n/a-n/a [Peer Reviewed Journal]

Full text available

View all versions
Citations Cited by
  • Title:
    Characterization of 13 microsatellite markers for Calochortus gunnisonii (Liliaceae) from Illumina MiSeq sequencing
  • Author: Fuller, Ryan S. ; Frietze, Seth ; Mcglaughlin, Mitchell E.
  • Subjects: Calochortus Gunnisonii ; Illumina ; Liliaceae ; Microsatellites ; Miseq
  • Is Part Of: Applications in Plant Sciences, August 2015, Vol.3(8), pp.n/a-n/a
  • Description: Premise of the study: Microsatellite primers were designed for Calochortus gunnisonii (Liliaceae), a montane lily species of the central and southern Rocky Mountains, using next-generation DNA sequencing. The markers will be used to investigate population structure, genetic diversity, and demographic history. Methods and Results: Thirteen polymorphic microsatellite loci were isolated from C. gunnisonii using Illumina MiSeq next-generation DNA sequencing and bioinformatic screening. The mean number of alleles per locus ranged from 4.15 to 5.92 (avg. = 4.97). Observed and expected heterozygosity ranged from 0.077 to 0.871 and 0.213 to 0.782, respectively. The primers were also tested for cross-species amplification value with C. flexuosus, C. nuttallii, C. kennedyi var. kennedyi, and C. subalpinus. Conclusions: These primers will be useful for genetic and evolutionary studies across C. gunnisonii's range within the southern and central Rocky Mountains. Furthermore, these markers have proven valuable for cross-species amplifications within Calochortus. ; p. .
  • Identifier: ISSN: 2168-0450 ; E-ISSN: 2168-0450 ; DOI: 10.3732/apps.1500051

Searching Remote Databases, Please Wait